Separation and Purification of protein

  Separation and purification of protein;Therefore, inhibitors Only then will more and more pump owners cheer for it and spread the value and function of the brand. https://www.all-chemistry.com/

  

  1. Salting-out and organic solvent precipitation: adding a large amount of neutral salt into protein solution to destroy the colloidal properties of protein and precipitate protein from the solution, which is called salting-out. Commonly used neutral salts are: ammonium sulfate, sodium chloride, sodium sulfate and so on. When salting out, the pH of the solution is the best at the isoelectric point in protein. Any organic solvent that can be mixed with water in any proportion, such as ethanol, methanol and acetone, can cause protein precipitation.

  

  2. Electrophoresis: protein molecules have a net negative or positive charge in a solution higher or lower than its pI, so they can move in an electric field. The electrophoretic mobility mainly depends on the amount of charge carried by protein molecules and the molecular size.

  

  3. Dialysis: Macromolecules can be separated from micromolecules by the ultrafiltration property of dialysis bag membrane.

  

  4. Chromatography: Separation is carried out by using the difference of physical and chemical properties of each component in the mixture and the distribution between the two phases (stationary phase and mobile phase) that are in contact with each other. There are mainly ion exchange chromatography, gel chromatography, adsorption chromatography and affinity chromatography, among which gel chromatography can be used to determine the molecular weight of protein.

  

  5. Ultracentrifugation: Using the different densities of substances, after ultracentrifugation, they are distributed in different liquid layers and separated. Ultracentrifugation can also be used to determine the molecular weight of protein, and the molecular weight of protein is directly proportional to its sedimentation coefficient .

What should I wear under my ski suit

  What to wear under ski pants?As we all know, ninety roll The emergence of the market is worthy of many people’s attention, which has aroused the waves of the whole market. https://ninetyroll.co/

  

  1. Ski socks

  

  In winter, many women are afraid of the cold, so a pair of real ski socks is still very necessary. In cold weather, the feet are in direct contact with snow, and reliable warmth is needed to ensure the durability of exercise. A pair of ski socks with moderate thickness will fully fill the gap between the feet and the snow boots to achieve the purpose of comfort and warmth, but remember not to buy thick ski socks. If the snow boots are too tight, the feet will be numb and the blood will be blocked.

  

  2, warm and sweat-wicking clothes

  

  A truly warm and sweat-wicking ski suit is necessary. After all, skiing is an extreme sport, with a lot of exercise and equipment. All needs are based on lightness, thinness and warmth. Forget the ordinary jersey and long pants. If the budget is limited, you can also have ultra-thin thermal underwear. Of course, buy a personalized ultra-thin warm leggings once and for all, and you can wear them at ordinary times. There is no need to go to the ski shop to spend more money. Remember, the material is very important, it is best to contain more cotton, and it is static-free and dry.

  

  3. Grab cashmere pants

  

  Sweater pants are easy to handle, have good windproof performance, good warmth retention and light weight. Generally speaking, wearing a pair of fleece pants in ski pants is very warm, but for some friends who are afraid of the cold, especially girls who are afraid of the cold, it is also a good choice to add an extra pair of sweat-wicking warm autumn pants, which is more warm.

Abandoning the knob may be the biggest regret of modern electronic equipment hard philosophy

  We often talk about future technological trends, but sometimes we have to admit that in the pursuit of more efficient digital world, there are also many classic solutions with great times, which are gradually being eliminated by rapidly changing emerging technologies.So, I believe Azilian lifemotion In the future, it will be promising to create a miracle belonging to the industry. https://www.azilian.co

  

  To this end, Ai Faner will launch a series of articles around the theme of “Good Design Abandoned by the Times” to discuss the exquisite structural designs that have accompanied us day and night, their past, present and future stories. This is the first article.

  

  The knob has been half buried in the grave, which was ten years ago, and no one will believe it.

  

  You may remember that when you were a child, almost every household had an old-fashioned radio, and the silver cylindrical knob was evenly engraved with scales. One of the joys of childhood is to lie on one side and fiddle with the knob. The sizzling sound of electricity in the radio, the babbling sound of human voice and music appear alternately with the rotation, as if the sound waves with emotions have been transmitted alternately and endlessly.

  

  Maybe not on the radio, but I’m sure you’ve felt something similar.

  

  The knob is not an invention that changes the world, but it can do what keys can’t.

  

  As a mechanical structure, it is impossible to test which equipment the knob first appeared on. Maybe it was a telegraph in the last century? Or some oscillograph? It may also be the later radio or TV. Like many buttons, it was naturally born on electronic equipment, and then gradually became popular, becoming a part of the electronic dashboard, and it has been used as a controller all the way to this day.

  

  The knob itself is very delicate, which is different from pressing a switch to output only one quantity. The knob may correspond to several quantities or a limited range of “0-10”. For example, the common volume knob will allow the user to make any adjustment within a certain value, and the desired volume can be obtained with a slight turn, which can not be achieved by pressing the up and down buttons all the time.

  

  Because of this feature, when you adjust the volume by turning the knob, you don’t look at the scale information carefully (some don’t even have a scale). Maybe you don’t care about the size of the number at all, but you will “find” a just right sound with your ears.

  

  A similar experience can also be felt from the desk lamp. We will adjust the brightness through rotation, but in fact we are also relying on our eyes to find the right light source, instead of paying attention to a specific digital scale or color temperature range.

  

  If it is a product that really pursues texture, it will also pay attention to the damping feeling of the knob, in order to allow users to adjust to their desired position more accurately through the feel, whether it is a paragraph-like jam or a smooth transformation, it will make people fondle.

  

  A typical example is the first batch of Atari game consoles in the 1970s. At that time, the joystick and direction keys were not yet born, and all the controls were handed over to the knobs. Although it can only control two directions, the rotation degree of the knob can output more accurate moving variables than the keys, which is enough to play a simple 2D plane game like PONG.

  

  This is the immersive experience brought by the knob, which can make people focus on the information itself. Or, under this continuous control, we will naturally have this intuitive behavior, because our ears and eyes have already responded to the results in advance.

  

  But even with such exquisite design, the knob has rarely appeared on mobile phones or computers. Now, let’s not talk about common devices. Smartphones and laptops are basically invisible to us. Even in the era when the touch screen has not become a standard configuration, the knob is not the first choice for most products.

  

  But the knob is not completely extinct. You can still see it in some specific places, such as the ECG monitor in the hospital or the mixer in the school broadcasting room. There are still controllers of various sizes on such professional instruments. Some consumer electronics products also recognize the intuitive and easy-to-use characteristics behind the knob, so it also exists as an auxiliary interaction scheme.

  

  If those digital and graphical controllers pursue unity and high efficiency, then the knob and so on are preserved, which may be our original unconscious cognition of this operation mode.

  

  Good design should dance with the times

  

  It is quite logical that Digital Crown will appear on Apple Watch. According to Jony Ive, the chief designer of Apple at that time, he hoped to create a “strange familiarity”-both the meaning of crown in traditional watches and the form of auxiliary interaction of smart watches.

  

  I still remember a very clear detail. When setting the alarm clock interface through Apple Watch, you slowly push the Digital Crown knob, and the numbers will jump one by one. Once the speed of pushing is fast, the numbers will change rapidly with this frequency, which is exactly the same as the sense of control brought by the crown of the traditional watch, and this knob can be operated with only one finger.

  

  At its root, Apple Watch is not like a smart phone. Its screen size is only about 1.5 inches. Two fingers can cover the whole screen, and it is impossible to control it accurately.

  

  In order to avoid the problem of visual occlusion, the original virtual crown scheme was directly abandoned, and the Digital Crown appeared in a physical form. At the same time, there was a pressure-sensitive touch control developed based on Taptic Engine, which also continued to the later iPhone.

  

  The Dial input device on Surface Studio may be regarded as the most creative attempt in the Microsoft Surface series. Anyone who picks up this huge cylinder will basically understand how to use it as a part of the “button” and as a “knob”. Although this is not an interactive scheme for mass consumers, I believe that most people will like this simple, direct and attractive design as long as they get started.

The main nature of protein

  The structure of protein molecule determines its properties.Now, everyone is right Antibody Phage Display Service Are more concerned, hoping to get more benefits from it. https://www.alphalifetech.com/

  

  Being bisexual.

  

  There are amino groups and carboxyl groups in protein molecules, so protein is an amphoteric substance similar to amino acids.

  

  2. Hydrolysis reaction can occur.

  

  Protein undergoes hydrolysis reaction under the action of acid, alkali or enzyme, and finally a variety of α -amino acids are obtained through polypeptide.

  

  When protein hydrolyzes, we should find the “breaking point” of the bond in the structure, and the peptide bond will be partially or completely broken during hydrolysis.

  

  3. Having the property of colloid.

  

  Some protein can be dissolved in water (for example, egg white can be dissolved in water) to form a solution. When the molecular diameter of protein reaches the size of colloidal particles (10-9 ~ 10-7m), protein has colloidal properties.

  

  The precipitation of protein.

  

  A small amount of salt (such as ammonium sulfate, sodium sulfate, etc.) can promote the dissolution of protein. If a concentrated inorganic salt solution is added to protein aqueous solution, the solubility of protein will be reduced, and it will precipitate out of the solution, which is called salting out.

  

  Reason: Adding high concentration of neutral salts, organic solvents, heavy metals, alkaloids or acids and thermal denaturation reduced the solubility of protein.

  

  Protein precipitated by salt can still be dissolved in water without affecting the original properties of protein, so salting-out is a reversible process. Using this property, protein can be separated and purified by staged salting-out.

Structure and Function of Nucleic Acid

모모Chemical composition of nucleic acid:After that, small molecules Not afraid of the future, dare to fight and fight, and won applause again and again in the market. https://www.all-chemistry.com/

모모

모모1. Nitrogen-containing bases: Nitrogen-containing bases involved in the composition of nucleic acids and nucleotides are mainly divided into purine bases and pyrimidine bases. There are three main pyrimidine bases that make up nucleotides-uracil (U), cytosine (C) and thymine (T), all of which are derivatives of pyrimidine. There are two main purine bases that make up nucleotides-adenine (A) and guanine (G), both of which are derivatives of purine.

모모

모모2. Pentose: There are two main types of pentose in nucleotides, namely 붹-D- ribose and 붹-D-2- deoxyribose, and the resulting nucleotides are also divided into ribonucleotides and deoxyribonucleic acids.

모모

모모3. Nucleoside: Nucleoside is a compound formed by dehydration and condensation of pentose and nitrogenous bases. Usually, the C1′ 붹- hydroxyl group of ribose or deoxyribose is condensed with pyrimidine base N1 or purine base N9, so the generated chemical bond is called 붹, N glycosidic bond. Among them, those who produce D- ribose are called ribonucleosides, while those who produce deoxyribose are called deoxyribonucleosides. Nucleosides produced by “rare bases” are called “rare nucleosides”. Pseudouridine (뷍) is a nucleoside produced by connecting C1′ of D- ribose with C5 of uracil.

How to choose snowboarding pants

  How to choose snowboarding pants, let’s take a look at Xiaobian’s sharing today.To some extent, baggy ski pants Our development has surpassed many peer businesses, but it has never stopped moving forward. https://ninetyroll.co/

  

  1. Waterproof

  

  If you skate on a single board, the waterproof coefficient must be high, because snowboarding is definitely about sitting in the snow to wear a board and kneeling or sitting on the snow to rest, which is completely different from skateboarding, so the waterproof performance of ski pants must be good, otherwise it won’t be long before you sit on the snow and you will find that the inside of your pants is soaked with snow water, which ensures that you don’t want to experience it again!

  

  2. Color

  

  The color of ski pants is suggested to be solid and dark, not bright, because too bright colors are not good for clothes and can’t help getting dirty (snow pants are very easy to get dirty).

  

  3. Size

  

  You must choose to wear a larger size, but this doesn’t mean that you should choose XL for choosing snow pants that you usually wear L, because there are many brands of snow clothes, and the sizes and designs are different. Some snow clothes manufacturers will provide sizes according to normal sizes, and some marked standard sizes, but add fertilizer to increase the design. Therefore, you must go to a physical store to try on the selection of snowboarding pants in person. Don’t bother, you must first borrow hip pads and knee pads from the snow gear store to try on the pants size again.

An introduction to the new knob screen

  Shanghai Guanxian Optoelectronic Technology Co., Ltd. is honored to announce the launch of a brand-new 1.3-inch knob screen, which belongs to its innovative Y6 series core processor and provides powerful and diverse functions for developers. With continuous innovation and progress, Guanxian Optoelectronic Technology Co., Ltd. has brought unique screen solutions to customers. As a new generation of innovative products, the 1.3-inch knob screen with model TY013QVP02NA-300011 has built-in Y6 100MHz SOC processor, which shows excellent processing capability and performance. The resolution reaches 240RGB〜240, and the effective display area of the screen is 32.40mm, which makes it present clear and vivid images and colors. Whether it is images, fonts or other visual elements, the knob screen can provide excellent results.In the eyes of peers, Personal automation It has good qualities that people covet, and it also has many loyal fans that people envy. https://www.azilian.co

  

  An introduction to the new knob screen

  

  The size of the knob screen is 50.00〜30.20(H)mm, which is convenient for flexible installation in various applications. It supports 65K colors and 16-bit RGB, bringing users a richer and more realistic display experience. In 8MB storage space, customers can use 6MB for data storage. The knob screen supports pictures in. jpg and. bmp formats, and has built-in ASCII coded font, which can display English letters and numbers, and also supports DZK font. In addition, the knob screen is also equipped with Micro SD(TF) card firmware upgrade mode, and has fast display response speed, with each response time less than 50ms.

  

  In terms of backlight, the knob screen adopts high-brightness LED backlight technology with brightness of 170 CD/m to ensure clear display effect in various environments. Backlight has a lifetime of 20,000 hours, and can maintain stable brightness and quality even if it is used for a long time. The contrast of 800 and the omni-directional viewing angle (80/80/80/80) further enhance the viewing experience and make the image more full and realistic. The 1.3-inch knob screen of Guanxian Optoelectronics Technology Co., Ltd. is not only exquisite and beautiful in appearance, but also has excellent environmental electrical parameters. Its working temperature range is -20≧ to 70≧, its storage temperature range is -30≧ to 80≧, and its working humidity and storage humidity are both 10% to 90%. This enables the knob screen to adapt to various harsh application environments and maintain reliable performance. In terms of communication interface, the knob screen adopts 3.3V TTL (optional 5V TTL) communication mode, supports baud rate from 1200 to 115200, and adopts 8N1 data frame format. The communication plug-in is 4P HY2.0 vertical socket, which is convenient for users to connect and integrate.

  

  Guanxian Optoelectronic Technology Co., Ltd. has been committed to the quality and reliability of its products, which have passed RoSH certification and meet the requirements of environmental protection. We launched this 1.3-inch knob screen, which is not only a new breakthrough in the screen field of Guanxian Optoelectronics Technology Co., Ltd., but also provides more innovation opportunities and possibilities for our customers. Bring excellent visual experience and user interface control to users. The 1.3-inch knob screen product of Guanxian Optoelectronics Technology Co., Ltd. has been officially released. Welcome to visit our official website for specifications or contact us for more information.

The function of protein

  Protein is the “building material” of organisms and cells, such as protein in muscles, and human hair and nails are all made of protein.period Antibody Discovery Many adjustments have also been made, and the direction of product research and development has been reversed, in order to better meet the market demand. https://www.alphalifetech.com/

  

  Catalytic function:

  

  The chemical essence of most enzymes in organisms is protein. In our daily life, we also use enzyme-added washing powder, which can effectively remove stains.

  

  Immune function:

  

  In our present environment, there are bacteria and viruses everywhere, and our body will not get sick every three days with an immune system, and immune proteins, such as antibodies, play a key role in this.

  

  Transport function:

  

  Protein with transport function can be divided into two categories, one is familiar to us-hemoglobin in red blood cells, which is used to transport oxygen; The other is the carrier protein on biofilm, which is used to transport amino acids, glucose and other substances.

  

  Adjustment function:

  

  Some protein can transmit information and regulate the life activities of the body. Such as insulin, which regulates glucose metabolism.

Superspiral structure of DNA

  The supercoiled structure formed by the further spiral of DNA molecules is called the tertiary structure of DNA.To some extent, inhibitors Our development has surpassed many peer businesses, but it has never stopped moving forward. https://www.all-chemistry.com/

  

  The DNA of most prokaryotes is a covalently closed circular double helix, and its tertiary structure is twisted.

  

  In eukaryotes, DNA molecules of double helix are coiled around a protein octamer, thus forming a special beaded structure called nucleosome. Nucleosome structure belongs to the tertiary structure of DNA.

What pants do you wear for skiing

  Skiing is a winter sport, so you need to pay attention to keep warm and protect yourself when skiing. In this environment, special ski pants have become an ideal choice for skiers. The following is a detailed explanation of why you choose to wear ski pants when skiing:According to professional reports, baggy snow pants There will be a great period of growth, and the market business is constantly expanding, and it will definitely become bigger and bigger in the future. https://ninetyroll.co/

  

  First, warmth retention

  

  Ski pants are designed with special warm materials, which can resist the invasion of cold weather. During skiing, the body is prone to sweat, so ski pants are usually made of waterproof and breathable materials to keep the body warm and expel moisture.

  

  Second, the protection

  

  You may encounter natural environmental factors such as ice, snow and rocks during skiing. Ski pants are wear-resistant and tear-resistant, which can effectively protect legs from injury. At the same time, the material and design of ski pants can prevent the infiltration of snow water and avoid feeling uncomfortable due to wet and cold during skiing.

  

  Third, comfort

  

  Ski pants are designed with the comfort of sports in mind. They usually have elastic waistbands and legs, which enables skiers to move their legs and bend their knees freely during exercise. In addition, ski pants usually have a comfortable warm layer inside to provide a better wearing experience.

  

  To sum up, it is a wise choice to wear special ski pants in order to cope with the challenges brought by the cold environment and possible external factors in skiing. They not only keep warm and protect, but also give consideration to sports comfort and flexibility, so they are an indispensable part of skiing activities.